Coding

Part:BBa_K2121003

Designed by: Jinny van Doorn   Group: iGEM16_Yale   (2016-10-20)


SPP1_GP35_Q38143

Coding sequence for beta recombinase protein homolog. For use in recombineering (eg Multiplex Automated Genome Engineering, MAGE) of Rhizobia species (ie S. meliloti and CIAT 899). Identified with BLASTp from homology to the SPP1 35 (Q38143) B. subtillus phage beta-like recombination protein. Derived from phage that selectively infects R.

Within Yale iGEM 2016, known as gBlock 14, amplified for cloning with primers priG 459, 460. Uniprot SPP1_GP35_Q38143 . E-value 4.90E-12


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None